New York City is experiencing a crime wave. Reporter April O'Neil discovers the thieves are ninja. As a street gang attacks April, she escapes into the sewers, where four big talking turtles defeat the thugs, and take April to their lair.
There April meets their mentor, a rat Splinter, who tells her his backstory as the Japanese ninja, Hamato Yoshi.
As the Turtles and April investigate the streets for the thieves' origins, they discover a group of robot ninjas whose uniform Splinter recognizes as belonging to the Foot Clan. Before April can expose them, she's kidnapped by the Shredder. The Turtles race to free her.